N202.1A

Main features:
N202.1A is a mammary carcinoma clone derived from a HER-2/neu transgenic mouse of FVB background (N#202 transgenic line). N202.1A cells express high levels of surface HER-2/neu. The expression of major histocompatibility complex class I glycoproteins (H-2^q) is low.
Culture conditions:
- Medium: Dulbecco's MEM + 20% FCS.
- Split when confluent, split ratio 1:2 - 1:4. Wash twice the monolayer with PBS, then detach cells with Trypsin-EDTA for 5' - 8' at 37°. Stop trypsin with DMEM+FCS and centrifuge.
- Freezing medium: 90% FCS (or NCS), 10% DMSO; cell density for freezing: 4 vials from a confluent 25 cm² flask.
- Thawing: seed one 25 cm² flask from one vial.
Main references:
- P.-L. Lollini, G. Nicoletti, L. Landuzzi, C. De Giovanni, I. Rossi, E. Di Carlo, P. Musiani, W.J. Muller, P. Nanni. Down regulation of Major Histocompatibility Complex class I expression in mammary carcinoma of HER-2/neu transgenic mice. Int. J. Cancer, 77: 937-941, 1998. [Medline]
- P. Nanni, S.M. Pupa, G. Nicoletti, C. De Giovanni, L. Landuzzi, I. Rossi, A. Astolfi, C. Ricci, R. De Vecchi, A.M. Invernizzi, E. Di Carlo, P. Musiani, G. Forni, S. Menard & P.-L. Lollini. p185 protein is required for tumor and anchorage-independent growth, not for cell proliferation of transgenic mammary carcinoma. Int. J. Cancer, 87: 186-194, 2000. [Medline]